Long-term exposure to low level of fluoride induces apoptosis via p53 pathway in lymphocytes of aluminium smelter workers

//Long-term exposure to low level of fluoride induces apoptosis via p53 pathway in lymphocytes of aluminium smelter workers

Long-term occupational exposure to low level of fluoride can induce oxidative stress and apoptosis in many cells, including lymphocyte. However, the underlying mechanism remains unclear. In the present study, the authors explored the potential oxidative stress and apoptosis of long-term occupational exposure to low level of fluoride in aluminium smelter workers. A total of 120 aluminium smelter workers were recruited in control, low-, middle-, and high-fluoride exposure groups with 30 workers for each group. The peripheral blood samples were collected, centrifuged, and isolated to obtain serum and lymphocyte suspensions. The air and serum fluoride concentrations were detected by fluoride ion-selective electrode method. The lymphocytic apoptosis rate, DNA damage, oxidative stress, and mRNA levels of p53, Bcl-2, and Bax were assessed by Annexin V/PI staining, comet assay, attenuated total reflectance Fourier transform infrared spectroscopy and real-time polymerase chain reaction, respectively. Results showed that the air and serum fluoride concentrations of fluoride-exposed groups were higher than those of the control group (p?

Authors: Wen P, Wei X, Liang G, Wang Y, Yang Y, Qin L, Pang W, Qin G, Li H, Jiang Y, Wu Q. ; Full Source: Environmental Science & Pollution Research International. 2019 Jan;26(3):2671-2680. doi: 10.1007/s11356-018-3726-z. Epub 2018 Nov 26.